The outbreak of African swine fever has seriously affected the balance of domestic pig breeding, leading to continued high prices of pigs, and the enthusiasm for resuming production and expansion of pig farms has also increased. Therefore, the ternary reserve group of pig farms has increased. This situation will bring greater hidden dangers to diarrhea in the farrowing room of pig farms. Because the primiparous sows have not been domesticated by the diarrhea virus, the IgA antibody is seriously insufficient, which greatly increases the probability of diarrhea in the farrowing room. This case is aimed at sows on a pig farm, using laboratory technology to detect their IgA antibodies to verify the combined live vaccine of transmissible gastroenteritis and porcine epidemic diarrhea (SD/L strain + LW/L strain) (referred to as "stomach"). The safety and effectiveness of the ventral double vaccine") under actual production conditions.

Disease situation of case pig farm

This pig farm has experienced diarrhea in the farrowing room and suffered heavy losses. Newborn piglets have symptoms such as vomiting, watery diarrhea, and dehydration and death of piglets within 3 days of age. The mortality rate of piglets within 5 days of age is nearly 100%. The morbidity rate has reached more than 50%, the disease lasted for about a week, and the loss was heavy. The current pig farm is stable and no diarrhea epidemic has occurred (this pig farm has not been immunized with diarrhea-related vaccines). Because the proportion of first-feet sows recently introduced in large numbers is as high as 60%, the current diarrhea epidemic risk and immune effect have not been evaluated, so an immunization test was carried out .

experiment material

vaccine

Porcine transmissible gastroenteritis and porcine epidemic diarrhea combined live vaccine (SD/L strain + LW/L strain) and transmissible gastroenteritis and porcine epidemic diarrhea combined inactivated vaccine (SD/L strain + LW) /L strain) provided by Luoyang Huizhong Biotechnology Co., Ltd.

Testing materials

Porcine epidemic diarrhea IgA-ELISA antibody detection reagents are provided and technically supported by a jointly accredited third-party laboratory.

Test plan

Take the sow’s blood and send it to a third-party laboratory for IgA antibody testing to analyze the risk of diarrhea, and use Huizhong Bio-Huifuning (Stomach-Abdominal Combination Live Vaccine) and Huifuan (Stomach-Abdominal Combination Vaccine) for IgA antibody testing. The pigs were tested for immunization. Three weeks after immunization, blood was collected to test for IgA antibodies. The antibody level confirmed the overall prevention and control ability of Huizhong Biological Huifuning and Huifuan (live + kill program) on pig farm diarrhea.

The test results of sow samples that have not been immunized with Huizhong Biology’s "Live + Kill" program are as follows:

The test results showed that the positive rate of IgA antibodies in the samples submitted by the pig farm was 13.33%, and the positive rate of IgA antibodies was too low. It was difficult to provide enough maternal antibodies for the piglets, and it was impossible to ensure that the piglets could resist epidemic diarrhea and infectious stomach. The invasion of enteritis, therefore, once the pig farm develops diarrhea in the production room, it will suffer heavy losses.

The results of sow samples submitted for inspection after immunization with Huizhong Biology’s "Live + Kill" program are as follows:

The positive rate of IgA antibody in sow samples after immunization with Huizhong Biology's "live + kill" program is 93.33%, which is much higher than the original positive rate before immunization, and can provide adequate maternal antibody protection for piglets in the farrowing room.